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  2. Field of view - Wikipedia

    en.wikipedia.org/wiki/Field_of_view

    Angular field of view is typically specified in degrees, while linear field of view is a ratio of lengths. For example, binoculars with a 5.8 degree (angular) field of view might be advertised as having a (linear) field of view of 102 mm per meter. As long as the FOV is less than about 10 degrees or so, the following approximation formulas ...

  3. Diffraction-limited system - Wikipedia

    en.wikipedia.org/wiki/Diffraction-limited_system

    An optical instrument is said to be diffraction-limited if it has reached this limit of resolution performance. Other factors may affect an optical system's performance, such as lens imperfections or aberrations, but these are caused by errors in the manufacture or calculation of a lens, whereas the diffraction limit is the maximum resolution ...

  4. Depth of field - Wikipedia

    en.wikipedia.org/wiki/Depth_of_field

    For cameras that can only focus on one object distance at a time, depth of field is the distance between the nearest and the farthest objects that are in acceptably sharp focus in the image. [ 1] ". Acceptably sharp focus" is defined using a property called the "circle of confusion". The depth of field can be determined by focal length ...

  5. Eye relief - Wikipedia

    en.wikipedia.org/wiki/Eye_relief

    Eye relief. The eye relief of an optical instrument (such as a telescope, a microscope, or binoculars) is the distance from the last surface of an eyepiece within which the user's eye can obtain the full viewing angle. If a viewer's eye is outside this distance, a reduced field of view will be obtained. The calculation of eye relief is complex ...

  6. Numerical aperture - Wikipedia

    en.wikipedia.org/wiki/Numerical_aperture

    In microscopy, NA generally refers to object-space numerical aperture unless otherwise noted. In microscopy, NA is important because it indicates the resolving power of a lens. The size of the finest detail that can be resolved (the resolution) is proportional to ⁠ λ / 2NA ⁠, where λ is the wavelength of the light. A lens with a larger ...

  7. Optical transfer function - Wikipedia

    en.wikipedia.org/wiki/Optical_transfer_function

    The two-dimensional optical transfer function at the focal plane can be calculated by integration of the 3D optical transfer function along the z-axis. Although the 3D transfer function of the wide-field microscope (b) is zero on the z-axis for z ≠ 0; its integral, the 2D optical transfer, reaching a maximum at x = y = 0.

  8. Point spread function - Wikipedia

    en.wikipedia.org/wiki/Point_spread_function

    By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...

  9. High-power field - Wikipedia

    en.wikipedia.org/wiki/High-power_field

    The area provides a reference unit, for example in reference ranges for urine tests. [3]Used for grading of soft tissue tumors: Grading, usually on a scale of I to III, is based on the degree of differentiation, the average number of mitoses per high-power field, cellularity, pleomorphism, and an estimate of the extent of necrosis (presumably a reflection of rate of growth).

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