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For cameras that can only focus on one object distance at a time, depth of field is the distance between the nearest and the farthest objects that are in acceptably sharp focus in the image. [ 1] ". Acceptably sharp focus" is defined using a property called the "circle of confusion". The depth of field can be determined by focal length ...
Modern stereomicroscope optical design. The stereo, stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it. The instrument uses two separate optical paths with two objectives ...
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen ... image quality rapidly degrades with increasing depth.
Confocal microscopy. Confocal microscopy, most frequently confocal laser scanning microscopy ( CLSM) or laser scanning confocal microscopy ( LSCM ), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [ 1 ]
Endomicroscopy. Endomicroscopy is a technique for obtaining histology -like images from inside the human body in real-time, [1] [2] [3] a process known as ‘optical biopsy’. [4] [5] It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use.
Depth of field depends on the focus distance, while depth of focus does not. Depth of focus can have two slightly different meanings. The first is the distance over which the image plane can be displaced while a single object plane remains in acceptably sharp focus; [1][2] [clarify] the second is the image-side conjugate of depth of field.
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