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Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria.
Salmonella species: red colonies, some with black centers. The agar itself will turn red due to the presence of Salmonella type colonies. Shigella species: red colonies. Coliforms: yellow to orange colonies. Pseudomonas aeruginosa: pink, flat, rough colonies. This type of colony can be easily mistaken for Salmonella due to the color similarities.
Rappaport-Vassiliadis soya peptone broth (RVS broth) is used as an enrichment growth medium for the isolation of Salmonella species. [1] It is not recommended for the enrichment of Salmonella Typhi or Paratyphi, which is inhibited due to the malachite green in RVS broth. [2] It is an alternative to selenite broth.
Salmonella spp appear to be yellow or colourless colonies, often with a dark centre. As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be ...
Salmonellosis is a symptomatic infection caused by bacteria of the Salmonella type. [1] It is the most common disease to be known as food poisoning (though the name refers to food-borne illness in general), these are defined as diseases, usually either infectious or toxic in nature, caused by agents that enter the body through the ingestion of food.
Biosafety level 4 (BSL-4) is the highest level of biosafety precautions, and is appropriate for work with agents that could easily be aerosol-transmitted within the laboratory and cause severe to fatal disease in humans for which there are no available vaccines or treatments.
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Selenite broth is used as a selective medium for the isolation of Salmonella species. [1] Selenite broth was originated by Leifson, [2] while observing good recovery of Salmonella spp. and reduced growth of fecal coliforms. Selenite broth is used as a selective enrichment for the cultivation of Salmonella spp. that may be present in small ...