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Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [ 1 ]
Mannitol is the primary ingredient of mannitol salt agar, a bacterial growth medium, and is used in others. Mannitol is used as a cutting agent [ 29 ] [ 30 ] in various drugs that are used intranasally ( snorted ), such as heroin and cocaine .
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Mannitol salt agar is also a selective and differential medium. The mannitol indicates organisms that ferment mannitol: mannitol fermentation produces lactic acid, lowering the pH and turning the plate yellow. The salt is to select for halophiles; organisms that cannot withstand a high salt content are unable to grow well.
Second, the isolate is cultured on mannitol salt agar, which is a selective medium with 7.5% NaCl that allows S. aureus to grow, producing yellow-colored colonies as a result of mannitol fermentation and subsequent drop in the medium's pH. [98] [99]
The liquid culture pasteur developed allowed for the visulization of promoting or inhibiting growth of specific bacteria. This same technique is utilized today through various mediums like Mannitol salt agar, a solid medium. Solid cultures were developed in 1881 when Robert Koch solidified the liquid media through the addition of agar [2]
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Agar is a gelatinous substance derived from seaweed. A cheap substitute for agar is guar gum, which can be used for the isolation and maintenance of thermophiles.