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Differentiated adipocytes in a 3T3-L1 cell line stained with Oil Red O. 3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions.
3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green .
This article lists protocols, categorized by the nearest layer in the Open Systems Interconnection model.This list is not exclusive to only the OSI protocol family.Many of these protocols are originally based on the Internet Protocol Suite (TCP/IP) and other models and they often do not fit neatly into OSI layers.
Reverse transfection is a technique for the transfer of genetic material into cells.As DNA is printed on a glass slide for the transfection process (the deliberate introduction of nucleic acids into cells) to occur before the addition of adherent cells, the order of addition of DNA and adherent cells is reverse that of conventional transfection. [1]
The PiggyBac (PB) transposon system employs a genetically engineered transposase enzyme to insert a gene into a cell's genome. It is built upon the natural PiggyBac (PB) transposable element (transposon), enabling the back and forth movement of genes between chromosomes and genetic vectors such as plasmids through a "cut and paste" mechanism.
Intracellular delivery is a fundamental technique in the study of biology and genetics, such as the use of DNA plasmid transfection to investigate protein function in living cells. [10] A wide range of approaches exist for performing intracellular delivery including biological, chemical and physical techniques that work through either membrane ...
Tissue nanotransfection (TNT) is an electroporation-based technique capable of gene and drug cargo delivery or transfection at the nanoscale. Furthermore, TNT is a scaffold-less tissue engineering (TE) technique that can be considered cell-only or tissue inducing depending on cellular or tissue level applications.
Hydrodynamic Delivery was developed as a way to insert genes without viral infection (transfection). The procedure requires a high-volume DNA solution to be inserted into the veins of the rodent using a high-pressure needle. [2] The volume of the DNA is typically 8-10% equal to 8-10% of the animal's body weight, and is injected within 5-7 seconds.