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Transcribed Image Text: DNA polymerase I has 5'-3' polymerase activity, 5'-3' exonuclease activity, and 3'-5' exonuclease activity necessary for DNA replication. Mutations in the gene that encodes DNA polymerase I may cause the enzyme to lose these activities.
Note that all or only a some steps may be used. 1: Synthesis is initiated on the 3-hydroxyl group of the primer. 2: Two strands of a duplex DNA molecule are separated. 3: DNA polymerase makes a DNA chain using an existing chain as a template. 4: A primer base-pairs with the complementary region of the DNA to be copied. 5: A primer base-pairs ...
Q: describe the function of Helicase, and DNA Polymerase in the DNA replication process. A: DNA helicases are fundamental during DNA replication since they separate double stranded DNA into… Q: Explain the complexities of DNA replication that make the process (a) bidirectional and (b)…
The last step requires DNA polymerase to synthesize a new strand of DNA that is complementary to the RNA strand in the 5’ to 3’ direction (Amplifying DNA: The Polymerase Chain Reaction, 2016). The forward and reverse primers are needed to start the replication process by providing the appropriate nucleotides to the new strand.
The proofreading function of DNA polymerase involves the recognitionof a _____ and the removal of a short segment of DNA in the _____ direction.a. missing base, 5′ to 3′b. base pair mismatch, 5′ to 3′c. missing base, 3′ to 5′d. base pair mismatch, 3′ to 5′
Part A How is a gene for a particular protein inserted into a plasmid? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. phosphate groups sticky ends an apurinic enzyme nitrogen-containing bases a recombinant DNA joined Submit cut ovide Feedback a donor DNA a restriction enzyme Request Answer When a gene has been obtained using Reset Help it is mixed ...
In DNA Replication, what is the function of Primase: works to connect discontinuous parts of DNA such as the nicks created by gyrase and the okazaki fragments O Synthesizes RNA on a DNA template to provide an available 3'-OH group to continue with DNA synthesis O binds at the origin and begins to unwind the helix O bind near the origin to ...
What is the function of DNA polymerase in DNA replication? to create replication bubbles by unravelling the parental strands of DNA to insert new bases during elongation, using the parent strand as a template O to create an RNA primer to initiate DNA replication to anneal (splice) smaller fragments of DNA to create one continuous strand O all of the above
1. DNA polymerase A. Short DNA sequences synthesized on the lagging strand 2. replication fork B. Replicated discontinuously in the 5' to 3' direction 3. Chromosome C. The structure made of DNA that carries genes 4. free nucleotides D. The Cs, Gs, Ts, & As ready to be added to growing chain 5. Topoisomerase F.
Enzyme function is critically important for the proper replication of DNA. Predict the consequence of a loss of function for each of the following enzymes a. DNA gyrase b. DNA polymerase III c. DNA ligase d. DNA polymerase I Solution Summary: The author explains the consequences of the loss of function of DNA gyrase.