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Epiboly in zebrafish is the first coordinated cell movement, beginning at the dome stage late in the blastula period and continuing throughout gastrulation. [3] At this point the zebrafish embryo contains three portions: an epithelial monolayer known as the enveloping layer (EVL), a yolk syncytial layer (YSL) which is a membrane-enclosed group of nuclei that lie on top of the yolk cell, and ...
This layer functions in protection by allowing the embryo to develop in a hypotonic solution so the cell will not burst. [5] Finally, the third set of blastomeres are the deep cells. These deep cells are located between the enveloping layer and the yolk syncytial layer and eventually give rise to the embryo proper. [1]
Zebrafish embryo 48 hours after fertilization. The arrows indicate four Rohon–Beard neurons. [1] Rohon–Beard cells are specialized primary neurons with mechanoreceptive properties. [2] [3] They occur during the embryonic stage of development and are found in the dorsal part of the spinal cord in fishes and amphibians.
This entire process is referred to as gene expression; it is the process by which the information in a gene, encoded as a sequence of bases in DNA, is converted into the structure of a protein. A Morpholino can modify splicing, block translation, or block other functional sites on RNA depending on the Morpholino's base sequence.
This generally involves the action of cytoplasmic determinants, located within parts of the fertilized egg, and of inductive signals emitted from signaling centers in the embryo. The early stages of regional specification do not generate functional differentiated cells, but cell populations committed to developing to a specific region or part ...
The process by which uniform gene expression becomes spatially and temporally differential is known as symmetry breaking. For example, in the case of embryonic Drosophila development, the genes nanos and bicoid are asymmetrically expressed in the oocyte because maternal cells deposit messenger RNA (mRNA) for these genes in the poles of the egg ...
VE-cadherin is indispensable for proper vascular development – there have been two transgenic mouse models of VE-cadherin deficiency, both embryonic lethal due to vascular defects. [ 10 ] [ 11 ] Further studies using one of these models revealed that although vasculogenesis occurred, nascent vessels collapsed or disassembled in the absence of ...
In animals, the process involves a sperm fusing with an ovum, which eventually leads to the development of an embryo. Depending on the animal species, the process can occur within the body of the female in internal fertilization, or outside in the case of external fertilization. The fertilized egg cell is known as the zygote. [2] [5]