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Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component that serves as a template for the telomere repeat.
The role of telomeres and telomerase in cell aging and cancer was established by scientists at biotechnology company Geron with the cloning of the RNA and catalytic components of human telomerase [9] and the development of a polymerase chain reaction (PCR) based assay for telomerase activity called the TRAP assay, which surveys telomerase ...
Since evidence has also shown that TERRA expression is regulated in a telomere-length-dependent manner, [3] it is believed that TERRA may play an important role along with telomerase to modulate the length of telomere repeats on chromosome ends. Generally speaking, cells with long telomeres exhibit greater TERRA expression while short telomeres ...
Telomerase RNA component, also known as TR, TER or TERC, is an ncRNA found in eukaryotes that is a component of telomerase, the enzyme used to extend telomeres. [ 3 ] [ 4 ] TERC serves as a template for telomere replication ( reverse transcription ) by telomerase.
Telomeres are shortened with every duplication of DNA, and must be lengthened again. He studies telomerase , the enzyme that copies the telomeric sequences and lengthens them. [ 8 ] The active site protein subunits of telomerase comprise a new class of reverse transcriptases , enzymes previously thought to be restricted to viruses and ...
The RNA component of telomerase anneals to the single stranded 3' end of the template DNA and contains 1.5 copies of the telomeric sequence. [93] Telomerase contains a protein subunit that is a reverse transcriptase called telomerase reverse transcriptase or TERT. TERT synthesizes DNA until the end of the template telomerase RNA and then ...
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The TERT subunit, an example of a reverse transcriptase, uses the RNA subunit to form the primer–template junction that allows telomerase to extend the 3' end of chromosome ends. The gradual decrease in size of telomeres as the result of many replications over a lifetime are thought to be associated with the effects of aging. [14]: 248–249
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