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Each strand in the double helix acts as a template for synthesis of a new, complementary strand. New DNA is made by enzymes called DNA polymerases, which require a template and a primer (starter) and synthesize DNA in the 5' to 3' direction. During DNA replication, one new strand (the leading strand) is made as a continuous piece.
Lesson 3: DNA replication. DNA replication and RNA transcription and translation. Leading and lagging strands in DNA replication. Speed and precision of DNA replication. Molecular structure of DNA. Molecular mechanism of DNA replication. Mode of DNA replication: Meselson-Stahl experiment. DNA proofreading and repair. Telomeres and telomerase.
A technique used to amplify, or make many copies of, a specific target region of DNA.
An in-depth looks at how transcription works. Initiation (promoters), elongation, and termination.
Review the structure and replication of DNA, including key concepts and processes involved.
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In E. coli, polymerase can replicate up to 700+ base pairs per second. Initially, one mistake occurs for every 10 million nucleotides, but proofreading reduces errors to one in a billion. This amazing cellular machinery keeps our DNA accurate and functional.
DNA, short for deoxyribonucleic acid, consists of nucleotides forming a double helix structure. Nucleotides contain a phosphate group, deoxyribose sugar, and a nitrogenous base. The bases, adenine, thymine, cytosine, and guanine, pair up through hydrogen bonds, creating the rungs of the DNA ladder.
Transcription involves rewriting genetic information from DNA to mRNA, with RNA polymerase playing a crucial role. In eukaryotic cells, DNA to mRNA transcription occurs within the nucleus, producing pre-mRNA.
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