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While some methods and protocols from human and mouse cell culture may apply to agricultural cellular materials, it has become clear that most do not. This is evidenced by the fact that established protocols for creating human and mouse embryonic stem cells have not succeeded in establishing ungulate embryonic stem cell lines. [16] [17] [18]
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Once the cells are plated, they are grown for 3–7 days under careful observation (while changing media each day) until desired confluence is reached. More recently, a study on In vitro generation of type-II pneumocytes initiated from human CD34(+) stem cells has been demonstrated the air-liquid interface cell culture method precisely. [3]
Current Protocols is a series of laboratory manuals for life scientists. The first title, Current Protocols in Molecular Biology, was established in 1987 by the founding editors Frederick M. Ausubel, Roger Brent, Robert Kingston, David Moore, Jon Seidman, Kevin Struhl, and John A. Smith of the Massachusetts General Hospital Department of Molecular Biology and the Harvard Medical School ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Nature Protocols, published by the Nature Publishing Group, is an on-line scientific journal publishing methods in a recipe-style format. The journal was launched in June 2006 and the content includes both classical methods and cutting-edge techniques relevant to the study of biological problems.
To obtain a high complexity library of ligation products that will ensure high resolution and depth of data, a sample of 20–25 million cells is required as input for Hi-C. [3] [4] Primary human samples, which may be available only in fewer cell numbers, could be used for standard Hi-C library preparation with as low as 1–5 million cells. [4]
It was established in 2013 and is published exclusively online by Cell Press. It is the official journal of the International Society for Stem Cell Research. The editor-in-chief is Martin Pera (Jackson Laboratory). According to the Journal Citation Reports, the journal has a 2020 impact factor of 7.765. [1]