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Lauryl tryptose broth is used for the most probable number test of coliforms in waters, effluent or sewage. It acts as a confirmation test for lactose fermentation with gas production. Sodium lauryl sulfate inhibits organisms other than coliforms. Formula in grams/litre (g/L) Tryptose: 20.0, Lactose : 5.0, Sodium chloride : 5.0,
Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color. The media inhibits growth of gram-positive organisms with crystal violet and bile salts, allowing for the selection and isolation of gram-negative bacteria. The media detects lactose fermentation by enteric bacteria with the pH indicator neutral red. [2]
Early in the development of microbiology, milk was used as a convenient, rich growth medium for propagating bacteria. The litmus in the medium acts as both a pH indicator and a redox (oxidation-reduction) indicator. The test itself tells whether the bacterium can ferment lactose, reduce litmus, form clots, form gas, or start peptonization. [2]
A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination. The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate ...
Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria.
Cystine tryptic agar (CTA), also known as cystine trypticase agar, [1] [2] is a growth medium used for the identification of microorganisms. [3]It can be used to determine if organisms can ferment various carbohydrates, including maltose, lactose, and sucrose.
Sorbitol-MacConkey agar is a variant of traditional MacConkey agar used in the detection of E. coli O157:H7. [1] Traditionally, MacConkey agar has been used to distinguish those bacteria that ferment lactose from those that do not.
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
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