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Fourth, probiotics must be safe for their intended use. The 2002 FAO/WHO guidelines recommend that, though bacteria may be generally recognized as safe (GRAS), the safety of the potential probiotic be assessed by the minimum required tests: [141] Assessment of certain metabolic activities (e.g. D-lactate production, bile salt deconjugation)
In microbiology, a colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions. Counting with colony-forming units requires culturing the microbes and counts only viable cells, in ...
An example of fermented milk, a dairy product L. acidophilus is commonly added to for probiotic effects. As stated in a journal from the American Dairy Science Association, "Lactobacillus acidophilus is a commercial strain and probiotic that is widely used in the dairy industry to obtain high-quality fermentation products."
The count represents the number of colony forming units (cfu) per g (or per ml) of the sample. A TVC is achieved by plating serial tenfold dilutions of the sample until between 30 and 300 colonies can be counted on a single plate. The reported count is the number of colonies counted multiplied by the dilution used for the counted plate
[74] [75] [76] As set by Food and Agriculture Organization (FAO) of the United Nations and the World Health Organization (WHO), the standard in order to be considered a health food with probiotic addition, the product should contain per gram at least 10 6-10 7 cfu of viable probiotic bacteria. [77]
The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. [1] The Miles and Misra method has been shown to be ...
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