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  2. Sticky and blunt ends - Wikipedia

    en.wikipedia.org/wiki/Sticky_and_blunt_ends

    The simplest DNA end of a double stranded molecule is called a blunt end. Blunt ends are also known as non-cohesive ends. In a blunt-ended molecule, both strands terminate in a base pair. Blunt ends are not always desired in biotechnology since when using a DNA ligase to join two molecules into one, the yield is significantly lower with blunt ...

  3. Lambda phage - Wikipedia

    en.wikipedia.org/wiki/Lambda_phage

    The repressor found in the phage lambda is a notable example of the level of control possible over gene expression by a very simple system. It forms a 'binary switch' with two genes under mutually exclusive expression, as discovered by Barbara J. Meyer .

  4. Ligation (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Ligation_(molecular_biology)

    Restriction enzymes can generate a wide variety of ends in the DNA they digest, but in cloning experiments most commonly-used restriction enzymes generate a 4-base single-stranded overhang called the sticky or cohesive end (exceptions include NdeI which generates a 2-base overhang, and those that generate blunt ends). These sticky ends can ...

  5. Lysogenic cycle - Wikipedia

    en.wikipedia.org/wiki/Lysogenic_cycle

    The prokaryotic cell is shown with its DNA, in green. 2. The bacteriophage attaches and releases its DNA, shown in red, into the prokaryotic cell. 3. The phage DNA then moves through the cell to the host's DNA. 4. The phage DNA integrates itself into the host cell's DNA, creating prophage. 5. The prophage then remains dormant until the host ...

  6. Integration host factor - Wikipedia

    en.wikipedia.org/wiki/Integration_host_factor

    When the Integration Host Factor was first discovered, it was only known for the site-specific recombination of bacteriophage. [4] This is all we knew for a while but through another article, we were able to find that with further research, IHF plays a key role in the scope of physiological processes of E. Coli, including site-specific recombination activities, phage packaging and partitioning ...

  7. Restriction enzyme - Wikipedia

    en.wikipedia.org/wiki/Restriction_enzyme

    These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. [ 4 ] [ 5 ] Inside a prokaryote , the restriction enzymes selectively cut up foreign DNA in a process called restriction digestion ; meanwhile, host DNA is protected by a modification enzyme (a methyltransferase ) that modifies the ...

  8. Joey Galloway: Indiana should bench QB Kurtis Rourke vs ... - AOL

    www.aol.com/joey-galloway-indiana-bench-qb...

    ESPN's Joey Galloway on Tuesday said Indiana football shouldn't play quarterback Kurtis Rourke vs. Ohio State to keep its CFP path intact.

  9. IGL@ - Wikipedia

    en.wikipedia.org/wiki/IGL@

    n/a Ensembl n/a n/a UniProt n a n/a RefSeq (mRNA) n/a n/a RefSeq (protein) n/a n/a Location (UCSC) n/a n/a PubMed search n/a Wikidata View/Edit Human Immunoglobulin lambda locus, also known as IGL@, is a region on the q arm of human chromosome 22, region 11.22 (22q11.22) that contains genes for the lambda light chains of antibodies (or immunoglobulins). Function Immunoglobulins recognize ...