Search results
Results from the WOW.Com Content Network
A sputum culture is a test to detect and identify bacteria [1] or fungi that infect the lungs or breathing passages. Sputum is a thick fluid produced in the lungs and in the adjacent airways. Normally, fresh morning sample is preferred for the bacteriological examination of sputum. [ 2 ]
The best sputum samples contain very little saliva, [2] as saliva contaminates the sample with oral bacteria. This is especially true for samples for laboratory testing in cytology or microbiology. Specimen adequacy is assessed by the laboratory technologists by examining a Gram stain or cytology stain of the sputum.
Mycobacteria Growth Indicator Tube (MGIT) samples in ultraviolet light. Emission of orange fluorescence indicates the presence of mycobacteria. The samples without fluorescence in this image still reflect some light from the others. Mycobacteria Growth Indicator Tube (MGIT) is intended for the culture, detection and recovery of mycobacteria.
The size of the loop determines the volume of liquid an inoculation loop can transfer. An early report of the use of an inoculation loop as an analytical tool was by O'Sullivan et al. [3] in a 1960 published protocol developed to improve methods for culturing urine samples. A 3mm diameter loop was used to deliver a consistent volume of urine ...
For example, different types of samples must be collected in appropriate tubes to maintain the integrity of the sample and stored at appropriate temperatures (usually 4 °C) to preserve the virus and prevent bacterial or fungal growth. Sometimes multiple sites may also be sampled. [citation needed] Types of samples include the following:
The medical history includes obtaining the symptoms of pulmonary TB: productive, prolonged cough of three or more weeks, chest pain, and hemoptysis.Systemic symptoms include low grade remittent fever, chills, night sweats, appetite loss, weight loss, easy fatiguability, and production of sputum that starts out mucoid but changes to purulent. [1]
Subsequently, aliquots of the sample are taken to test for the antimicrobial activity of a specific drug or protein (antimicrobial peptides). Liquid cultures of the cyanobacterium Synechococcus PCC 7002. Static liquid cultures may be used as an alternative. These cultures are not shaken, and they provide the microbes with an oxygen gradient. [6]
To collect the sample, the swab is inserted in the nostril and gently moved forward into the nasopharynx, a region of the pharynx that covers the roof of the mouth. [9] The swab is then rotated for a specified period of time to collect secretions, then the swab is removed and placed into a sterile viral transport media , which preserves the ...