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  2. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.

  3. Spin column-based nucleic acid purification - Wikipedia

    en.wikipedia.org/wiki/Spin_column-based_nucleic...

    The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...

  4. Nucleic acid methods - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_methods

    DNA sequencing; Expression cloning; Fluorescence in situ hybridization; Lab-on-a-chip; Comparison of nucleic acid simulation software; Northern blot; Nuclear run-on assay; Radioactivity in the life sciences; Southern blot; Differential centrifugation (sucrose gradient) Toeprinting assay; Several bioinformatics methods, as seen in list of RNA ...

  5. DNA sequencing - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing

    DNA sequencing is the process of determining the nucleic ... laborious methods based on two-dimensional chromatography. ... DNA extraction will yield a DNA sample ...

  6. Agarose gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Agarose_gel_electrophoresis

    Estimation of the DNA concentration by comparing the intensity of the nucleic acid band with the corresponding band of the size marker. [34] Analysis of products of a polymerase chain reaction (PCR), e.g., in molecular genetic diagnosis or genetic fingerprinting; Separation of DNA fragments for extraction and purification.

  7. DNA profiling - Wikipedia

    en.wikipedia.org/wiki/DNA_profiling

    The most common methods of DNA extraction include organic extraction (also called phenol–chloroform extraction), [17] Chelex extraction, and solid-phase extraction. Differential extraction is a modified version of extraction in which DNA from two different types of cells can be separated from each other before being purified from the solution ...

  8. The process of recreating the face is one of the few options available for investigators, who have been trying to use DNA analysis in the five days since the horrifying murder.

  9. Solid-phase extraction - Wikipedia

    en.wikipedia.org/wiki/Solid-phase_extraction

    SPE is in fact a method of chromatography, in the sense of having a mobile phase, carrying mixtures through a stationary phase, packed inside a column.The chromatographic process is harnessed to create a solid-liquid extractive technique—allowing separation of a mixture of components by taking advantage of large differences between the solid and liquid phase K eq, or equilibrium constant ...

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