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Paper chromatography is an analytical method used to separate coloured chemicals or substances. [1] It is now primarily used as a teaching tool, having been replaced in the laboratory by other chromatography methods such as thin-layer chromatography (TLC). The setup has three components.
Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2]
Thin layer chromatography. Thin-layer chromatography (TLC) is a widely employed laboratory technique used to separate different biochemicals on the basis of their relative attractions to the stationary and mobile phases. It is similar to paper chromatography.
[5] [6] However, flat-bottom chambers and horizontal-development chambers are also used for specific compounds. A general mechanism for the HPTLC device goes as follows. [5] A fitted filter paper is placed in the rear trough of the chamber and the mobile phase is poured through the rear trough to ensure complete solvent absorption of the filter ...
Chromatography separates dissolved substances by different interaction with (i.e., travel through) a material. High-performance liquid chromatography (HPLC) Thin-layer chromatography (TLC) Countercurrent chromatography (CCC) Droplet countercurrent chromatography (DCC) Paper chromatography; Ion chromatography; Size-exclusion chromatography (SEC)
The earliest form of 2D-chromatography came in the form of a multi-step TLC separation in which a thin sheet of cellulose is used first with one solvent in one direction, then, after the paper has been dried, another solvent is run in a direction at right angles to the first.
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The retardation factor, R F, is commonly used in paper chromatography and thin layer chromatography (TLC) for analyzing and comparing different substances. It can be mathematically described by the following ratio: [2]
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