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Currently there are over 40 different application areas that cellomics is used in, including the analysis of 3-D cell models, angiogenesis, and cell-signalling. Originally a tool used by the pharmaceutical industry for screening, cellomics has now expanded into academia to better understand cell function in the context of the cell. [ 5 ]
In cryo-sectioning, frozen blocks of cells or tissue are sectioned into thin samples with a cryo-microtome. [11] In FIB-milling, plunge-frozen samples are exposed to a focused beam of ions, typically gallium , that precisely whittle away material from the top and bottom of a sample, leaving a thin lamella suitable for cryoET imaging.
Amira (ah-MEER-ah) is a software platform for visualization, processing, and analysis of 3D and 4D data. It is being actively developed by Thermo Fisher Scientific in collaboration with the Zuse Institute Berlin (ZIB), and commercially distributed by Thermo Fisher Scientific — together with its sister software Avizo.
Avizo (pronounce: ‘a-VEE-zo’) is a general-purpose commercial software application for scientific and industrial data visualization and analysis.. Avizo is developed by Thermo Fisher Scientific and was originally designed and developed by the Visualization and Data Analysis Group [1] at Zuse Institute Berlin (ZIB) under the name Amira.
More than 50 years ago, MSI was introduced using secondary ion mass spectrometry (SIMS) to study semiconductor surfaces by Castaing and Slodzian. [5] However, it was the pioneering work of Richard Caprioli and colleagues in the late 1990s, demonstrating how matrix-assisted laser desorption/ionization (MALDI) could be applied to visualize large biomolecules (as proteins and lipids) in cells and ...
A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full cell system, thus reducing the complex interactions typically found when working in a whole cell. [1]
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A bioassay is an analytical method to determine the potency or effect of a substance by its effect on living animals or plants (in vivo), or on living cells or tissues (in vitro). [1] [2] A bioassay can be either quantal or quantitative, direct or indirect. [3] If the measured response is binary, the assay is quantal; if not, it is quantitative ...