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Central slice through a tomogram of an intact Bdellovibrio bacteriovorus cell. Scale bar 200 nm. In electron microscopy (EM), samples are imaged in a high vacuum.Such a vacuum is incompatible with biological samples such as cells; the water would boil off, and the difference in pressure would explode the cell.
Energy-filtered transmission electron microscopy (EFTEM) is a technique used in transmission electron microscopy, in which only electrons of particular kinetic energies are used to form the image or diffraction pattern. The technique can be used to aid chemical analysis of the sample in conjunction with complementary techniques such as electron ...
A TEM image of a cluster of poliovirus.The polio virus is 30 nm in diameter. [1] Operating principle of a transmission electron microscope. Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image.
The pixels are typically wide and thick e.g. 150 x 150 x 500 μm for the electron microscope pixel array detector (EMPAD) described by Tate et al. [11] This large pixel size allows each pixel to fully absorb high-energy electrons, enabling high dynamic range. However, the large pixel size limits the number of pixels that can be incorporated ...
Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same phase. Cell synchrony is a vital process in the study of cells progressing through the cell cycle as it allows population-wide data to be collected rather than relying solely on single-cell experiments.
Avizo (pronounce: ‘a-VEE-zo’) is a general-purpose commercial software application for scientific and industrial data visualization and analysis.. Avizo is developed by Thermo Fisher Scientific and was originally designed and developed by the Visualization and Data Analysis Group [1] at Zuse Institute Berlin (ZIB) under the name Amira.
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Cell–cell interaction refers to the direct interactions between cell surfaces that play a crucial role in the development and function of multicellular organisms. These interactions allow cells to communicate with each other in response to changes in their microenvironment. This ability to send and receive signals is essential for the ...