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Proteus species do not usually ferment lactose. Similar to other members of the Enterobacterales order, bacteria from the Proteus genus are glucose fermenting, oxidase-negative, catalase-positive, and nitrate-positive. Glucose fermentation in this species can be demonstrated through the triple sugar iron test.
Proteus vulgaris is a rod-shaped, ... but does not ferment mannitol or lactose. ... test and is also an extremely motile organism.
Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color. The media inhibits growth of gram-positive organisms with crystal violet and bile salts, allowing for the selection and isolation of gram-negative bacteria. The media detects lactose fermentation by enteric bacteria with the pH indicator neutral red. [2]
Proteus mirabilis appears as Gram-negative rods after Gram staining under bright-field microscopy with 1000 times magnification. Proteus mirabilis is a Gram-negative, facultatively anaerobic, rod-shaped bacterium. It shows swarming motility and urease activity. P. mirabilis causes 90% of all Proteus infections in humans.
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
CLED agar – cysteine, lactose, electrolyte-deficient agar is used to isolate and differentiate urinary tract bacteria, since it inhibits Proteus species swarming and can distinguish between lactose fermenters and nonfermenters. Granada medium is used to isolate and differentiate group B Streptococcus, Streptococcus agalactiae from clinical ...
A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination. The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate ...
CLED agar (cystine–lactose–electrolyte-deficient agar or medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary microbes. It contains cystine and lactose and is electrolyte -deficient; the latter trait prevents the swarming of Proteus species.
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