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Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel. TGGE and DGGE can be applied to nucleic acids such as DNA and RNA, and (less commonly) proteins.
In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]
Aversive agents—primarily bitterants and pungent agents—are often used to produce an unpleasant flavor. For example, the bitterant denatonium might be added to food used in a laboratory, where such food is not intended for human consumption. [1] A poisonous substance may be added as an even more powerful deterrent.
The war between two top survival shelter firms has encompassed court battles, accusations of arson and murder, and wild tales of FBI double agents. Inside the bitter feud roiling the doomsday ...
Two Indian chemical companies have been indicted for allegedly importing ingredients for the highly addictive opioid fentanyl into the United States and Mexico, the U.S. Department of Justice said ...
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An alternative source of free radicals is riboflavin, which generated free radicals in a photochemical reaction. TEMED (N, N, N′, N′-tetramethylethylenediamine) (C 6 H 16 N 2; mW: 116.21) stabilizes free radicals and improves polymerization. The rate of polymerisation and the properties of the resulting gel depend on the concentrations of ...
Letters laced with infectious anthrax were concurrently delivered to news media offices and the U.S Congress, alongside an ambiguously related case in Chile. The letters killed 5. Bruce Edwards Ivins [6] 2003 2003 ricin letters: Ricin: 0 0 United States: Two ricin-laden letters were found on two separate occasions between October and November 2003.