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Affinity chromatography does not require the molecular weight, charge, hydrophobicity, or other physical properties of the analyte of interest to be known, although knowledge of its binding properties is useful in the design of a separation protocol. [5]
Triose phosphate isomerase is a dimer of identical subunits, each of which is made up of about 250 amino acid residues. The three-dimensional structure of a subunit contains eight α-helices on the outside and eight parallel β-strands on the inside.
This is termed as immobilized metal ion affinity chromatography (IMAC), as originally introduced in 1975 under the name metal chelate affinity chromatography. [3] Subsequent studies have revealed that among amino acids constituting proteins, histidine is strongly involved in the coordination complex with metal ions. [ 4 ]
BCCP (Biotin Carboxyl Carrier Protein), a protein domain biotinylated by BirA enabling recognition by streptavidin; BromoTag, a "bump-and-hole" mutated version of the second bromodomain of Brd4, Brd4-BD2 L387A, that can be highly selectively bound by tag-specific PROTAC degrader AGB1 to form a ternary complex between the "BromoTagged" protein and the E3 ligase VHL, leading to ubiquitination of ...
In biochemistry, medicine, and related sciences, inositol generally refers to myo-inositol (formerly meso-inositol), the most important stereoisomer of the chemical compound cyclohexane-1,2,3,4,5,6-hexol. Its formula is C 6 H 12 O 6; the molecule has a ring of six carbon atoms, each with an hydrogen atom and a hydroxyl group (–OH).
Microscopy of keratin filaments inside cells. Keratin (/ ˈ k ɛr ə t ɪ n / [1] [2]) is one of a family of structural fibrous proteins also known as scleroproteins.It is the key structural material making up scales, hair, nails, feathers, horns, claws, hooves, and the outer layer of skin in vertebrates.
“A lot of people always ask me how I do gymnastics with such long nails. To tell you the truth, they actually help me with my technique,” Chiles, 23, recently told the Associated Press ...
Uridine diphosphate, abbreviated UDP, is an organic compound.It is an ester of pyrophosphoric acid with the nucleoside uridine.UDP consists of the pyrophosphate group, the pentose sugar ribose, and the nucleobase uracil.