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The Michaelis constant is defined as the concentration of substrate at which the reaction rate is half of . [6] Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions.
Eadie–Hofstee plot of v against v/a for Michaelis–Menten kinetics. In biochemistry, an Eadie–Hofstee plot (or Eadie–Hofstee diagram) is a graphical representation of the Michaelis–Menten equation in enzyme kinetics. It has been known by various different names, including Eadie plot, Hofstee plot and Augustinsson plot.
When used to model enzyme rates in vivo , for example, to model a metabolic pathway, this representation is inadequate because under these conditions product is present. As a result, when building computer models of metabolism [ 1 ] or other enzymatic processes, it is better to use the reversible form of the Michaelis–Menten equation.
A plot depicting the initial reaction rate versus substrate concentration as modeled by the Michaelis-Menten equation (solid line) and the Haldane equation for substrate inhibition (dotted line). One of the most well known equations to describe single-substrate enzyme kinetics is the Michaelis-Menten equation.
The best known plots of the Michaelis–Menten equation, including the double-reciprocal plot of / against /, [2] the Hanes plot of / against , [3] and the Eadie–Hofstee plot [4] [5] of against / are all plots in observation space, with each observation represented by a point, and the parameters determined from the slope and intercepts of the lines that result.
In the Michaelis-Menten model, the enzyme binds to the substrate yielding an enzyme substrate complex, which can either go backwards by dissociating or go forward by forming a product. [2] The dissociation rate constant is defined using K off. [2] The Michaelis-Menten constant is denoted by K m and is represented by the equation K m = (K off ...
Hanes plot of a/v against a for Michaelis–Menten kinetics In biochemistry , a Hanes–Woolf plot , Hanes plot , or plot of a / v {\displaystyle a/v} against a {\displaystyle a} is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration a {\displaystyle a} to the reaction velocity v ...
This notation demonstrates that similar to the Michaelis–Menten equation, where the rate of reaction depends on the percent of the enzyme population interacting with substrate, the effect of the inhibitor is a result of the percent of the enzyme population interacting with inhibitor.