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A quarter of the world's SARS-CoV-2 viral genomes were sequenced with nanopore devices. The technology offers an important tool for combating antimicrobial resistance. [11] In 2020, China-based Qitan Technology launched its nanopore single-molecule gene sequencer, [12] while in 2024 MGI Tech launched its own products. [13]
The technique adapts the principle of resistive pulse sensing, which monitors current flow through an aperture, combined with the use of tunable nanopore technology, allowing the passage of ionic current and particles to be regulated by adjusting the pore size.
A nanopore is a pore of nanometer size. It may, for example, be created by a pore-forming protein or as a hole in synthetic materials such as silicon or graphene. When a nanopore is present in an electrically insulating membrane, it can be used as a single-molecule detector.
The Institute of Nano Science and Technology (INST) is an autonomous research institution of Department of Science and Technology (India), under the Society Registration Act, 1960, [1] under the umbrella of national mission on Nano Science and Technology (NANO MISSION)", [2] which aims to promote growth and outreach of nanoscience and technology for the benefit of country. [3]
Sequencing technologies with a different approach than second-generation platforms were first described as "third-generation" in 2008–2009. [4]There are several companies currently at the heart of third generation sequencing technology development, namely, Pacific Biosciences, Oxford Nanopore Technology, Quantapore (CA-USA), and Stratos (WA-USA).
The focus of the Indian Institute of Nano Science & Technology will be research and development of nano materials. [3] Nano Park is the first of its kind in India and supports the development of medical devices, diagnostic kits and pharmaceuticals. [4]
[32] [33] A mixture of different sequencing platforms are used for clinical viral metagenomics, the most common being instruments from Illumina and Oxford Nanopore Technologies. There are also several different protocols, both for wet lab work and for bioinformatic analysis, that are in use. [34]
Pore-C workflow. Many methods to characterize the 3D genome are variations on 3C technology. [5] Like other 3C-based technologies, [5] Pore-C seeks to characterize the architecture of the 3D genome by determining which genomic loci are in close spatial proximity (within ~200 nm). [2]