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The colloidal gold protein assay is a highly sensitive biochemical assay for determining the total concentration of protein in a solution (~0.1 ng/μL to 200 ng/μL). [1] It was first described in 1987 by two groups who used commercially available "Aurodye" colloidal gold solutions.
It is similar to the ristocetin cofactor assay but has the added benefit in that it helps in the diagnosis of type 2B/pseudo von Willebrand disease (vWD) and Bernard–Soulier syndrome because it uses patient's live endogenous platelets, whereas ristocetin cofactor assay tests the function of only the vWF and not the platelets. Ristocetin ...
Within the field of molecular biology, a protein-fragment complementation assay, or PCA, is a method for the identification and quantification of protein–protein interactions. In the PCA, the proteins of interest ("bait" and "prey") are each covalently linked to fragments of a third protein (e.g. DHFR, which acts as a "reporter").
This assay is one of the fastest assays performed on proteins. [12] The total time it takes to set up and complete the assay is under 30 minutes. [13] The entire experiment is done at room temperature. The Bradford protein assay can measure protein quantities as little as 1 to 20 μg. [14] It is an extremely sensitive technique.
Type I collagen is the most abundant collagen of the human body, consisting of around 90% of the body's total collagen in vertebrates. Due to this, it is also the most abundant protein type found in all vertebrates. Type I forms large, eosinophilic fibers known as collagen fibers, which make up most of the rope-like dense connective tissue in ...
Total complement activity ... Alternative pathway hemolytic assay ("AH50") can be used in conjunction to indicate if there is a terminal pathway deficiency (CH50 and ...
The C-terminal telopeptide (CTX), also known as carboxy-terminal collagen crosslinks, is the C-terminal telopeptide of fibrillar collagens such as collagen type I and type II. It is used as a biomarker in the serum to measure the rate of bone turnover .
The collagen gel contraction assay is an in vitro model of wound contraction. It is performed using the dermal equivalent model, ...