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The glycocholic acid breath test involves the administration of the bile acid 14 C glycocholic acid, and the detection of 14 CO 2, which would be elevated in bacterial overgrowth. [citation needed] However, some physicians suggest that if the suspicion of bacterial overgrowth is high enough, the best diagnostic test is a trial of treatment.
Coliform bacteria include genera that originate in feces (e.g. Escherichia, Enterobacter, Klebsiella, Citrobacter). The fecal coliform assay is intended to be an indicator of fecal contamination; more specifically of E. coli which is an indicator microorganism for other pathogens that may be present in feces.
By testing for coliforms, especially the well known Escherichia coli (E. coli), which is a thermotolerant coliform, one can determine if the water has possibly been exposed to fecal contamination; that is, whether it has come in contact with human or animal feces. It is important to know this because many disease-causing organisms are ...
While many Escherichia are commensal members of the gut microbiota, certain strains of some species, most notably the pathogenic serotypes of E. coli, are human pathogens, [7] and are the most common cause of urinary tract infections, [8] significant sources of gastrointestinal disease, ranging from simple diarrhea to dysentery-like conditions, [3] as well as a wide range of other pathogenic ...
The solution remaining yellow (pH = 6.2 or above) indicates a negative test, meaning the butanediol fermentation is used. The VP test uses alpha-naphthol and potassium hydroxide to test for the presence of acetylmethylcarbinol (acetoin), an intermediate of the 2,3-butanediol fermentation pathway. After adding both reagents, the tube is shaken ...
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Escherichia coli (E. coli) can be distinguished from most other coliforms by its ability to ferment lactose at 44 °C in the fecal coliform test, and by its growth and color reaction on certain types of culture media. When cultured on an eosin methylene blue (EMB) plate, a positive result for E. coli is metallic green colonies on a dark purple ...
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