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Although many microtechniques can be used in both plant and animal micro experiments. Some methods may differ from itself when employed in different field. Three commonly used preparation ways used in zoological micro observations can be concluded as paraffin method, celloidin method, freezing method, and miscellaneous techniques.
The International Collection of Microorganisms from Plants (ICMP) is a major international culture collection of live bacteria, fungi, and chromists based in Auckland, New Zealand. [ 1 ] The ICMP had its origin in 1952 as the personal collection of plant pathogenic bacteria and rhizobia of Dr Douglas W. Dye .
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Currently, there are several reports on standardisation of protocol for the Agrobacterium-mediated transformation. The effect of different parameters such as infection time, acetosyringone, DTT, and cysteine have been studied in soybean (Glycine max). [18] Possible plant compounds that initiate Agrobacterium to infect plant cells: [19]
Current Protocols is a series of laboratory manuals for life scientists. The first title, Current Protocols in Molecular Biology, was established in 1987 by the founding editors Frederick M. Ausubel, Roger Brent, Robert Kingston, David Moore, Jon Seidman, Kevin Struhl, and John A. Smith of the Massachusetts General Hospital Department of Molecular Biology and the Harvard Medical School ...
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is the most popular plant growth medium used in the laboratories worldwide for cultivation of plant cell culture on agar. MS0 was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS ...
Springer Protocols was a database of life sciences protocols published by Springer Science+Business Media. It replaced BioMed Protocols, a Humana Press database, in January 2008, and was deactivated on 25 July 2018. The protocols were then available on the SpringerLink website. [1]
Simplified Pressure-Volume Curve. A more advance method that uses the pressure bomb in plant physiology is pressure-volume curves analysis or p-v curve. Through this method one measures the changes in leaf or stem water potential and relative water content to isolate the underlying components of total leaf or stem water potential. [7]