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While the use of fluorescent proteins was once limited to the green fluorescent protein , in recent years many other fluorescent proteins have been cloned. Unlike GFPs, which are derived from the luminescent jellyfish Aequorea victoria, fluorescent proteins derived from anthozoa, including Eos, emit fluorescence in the red spectral range.
Dronpa is a reversibly switchable photoactivatable fluorescent protein that is 2.5 times as bright as EGFP. [1] [2] Dronpa gets switched off by strong illumination with 488 nm (blue) light and this can be reversed by weak 405 nm UV light. [1] A single dronpa molecule can be switched on and off over 100 times. [3]
Unlike other fluorescent proteins, PAFPs can be used as selective optical markers. An entirely labeled cell can be followed to assess cell division, migration, and morphology. Very small volumes containing PAFPs can be activated with a laser. In these cases, protein trafficking, diffusion, and turnover can be assessed.
Nucleic acids play important roles as cellular information storage and gene regulation machinery. In efforts to regulate this machinery by light, DNA and RNA have been modified with photocleavable groups at the backbone (in an approach called ‘statistical backbone caging’; the protection groups react mainly with backbone phosphate groups).
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As an RFP, mCherry was derived from DsRed of Discosoma sea anemones, unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish. [1]
The last image we have of Patrick Cagey is of his first moments as a free man. He has just walked out of a 30-day drug treatment center in Georgetown, Kentucky, dressed in gym clothes and carrying a Nike duffel bag.
Atlanta is sending De'Andre Hunter to Cleveland. And Philadelphia picked up Jared Butler and draft picks for Reggie Jackson. Read what you missed:
Study Group (Korean: 스터디그룹) is a South Korean television series co-written by Eom Seon-ho and Oh Bo-hyun, directed by Lee Jang-hoon and Yoo Beom-sang, and starring Hwang Min-hyun, Han Ji-eun, Cha Woo-min, Lee Jong-hyun, Shin Su-hyun, Yoon Sang-jeong, and Gong Do-yu.