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2. Relative fluorescence units - Wikipedia

   en.wikipedia.org/wiki/Relative_fluorescence_units

   The RFU measurements are used, for DNA profiling, in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labeled with a fluorescent reporter which permits detection only after ...

3. Primer (molecular biology) - Wikipedia

   en.wikipedia.org/wiki/Primer_(molecular_biology)

   The free NCBI tool Primer-BLAST integrates primer design and BLAST search into one application, [6] as do commercial software products such as ePrime and Beacon Designer. Computer simulations of theoretical PCR results ( Electronic PCR ) may be performed to assist in primer design by giving melting and annealing temperatures, etc. [ 7 ]

4. Cystic fibrosis transmembrane conductance regulator - Wikipedia

   en.wikipedia.org/wiki/Cystic_fibrosis_trans...

   1080 12638 Ensembl ENSG00000001626 ENSMUSG00000041301 UniProt P13569 P26361 RefSeq (mRNA) NM_000492 NM_021050 RefSeq (protein) NP_000483 NP_066388 Location (UCSC) Chr 7: 117.29 – 117.72 Mb Chr 6: 18.17 – 18.32 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Cystic fibrosis transmembrane conductance regulator (CFTСR) is a membrane protein and anion channel in vertebrates that is ...

5. Polymerase chain reaction - Wikipedia

   en.wikipedia.org/wiki/Polymerase_chain_reaction

   A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

6. Luciferase - Wikipedia

   en.wikipedia.org/wiki/Luciferase

   In the absence of GFP, this energy would be released as a photon of blue light (peak emission wavelength 482 nm). However, due to the closely associated GFP, the energy released by the luciferase is instead coupled through resonance energy transfer to the fluorophore of the GFP, and is subsequently released as a photon of green light (peak ...

7. Primer binding site - Wikipedia

   en.wikipedia.org/wiki/Primer_binding_site

   A primer binding site is a region of a nucleotide sequence where an RNA or DNA single-stranded primer binds to start replication. The primer binding site is on one of the two complementary strands of a double-stranded nucleotide polymer, in the strand which is to be copied, or is within a single-stranded nucleotide polymer sequence. [2]

8. Escherichia coli in molecular biology - Wikipedia

   en.wikipedia.org/wiki/Escherichia_coli_in...

   Escherichia coli is one of the most diverse bacterial species, with several pathogenic strains with different symptoms and with only 20% of the genome common to all strains. [1] Furthermore, from the evolutionary point of view, the members of genus Shigella ( dysenteriae , flexneri , boydii , sonnei ) are actually E. coli strains "in disguise ...