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Template-switching polymerase chain reaction (TS-PCR) is a method of reverse transcription and polymerase chain reaction (PCR) amplification that relies on a natural PCR primer sequence at the polyadenylation site, also known as the poly(A) tail, and adds a second primer through the activity of murine leukemia virus reverse transcriptase. [1]
OLIGO Primer Analysis Software is a software for DNA primer design. [ 1 ] [ 2 ] The first paper describing this software was published in 1989. [ 3 ] The program is a real time PCR primer and probe search and analysis tool.
A key activity for Bio-Synthesis was to synthesize large number of PCR primer thus assisting and solidifying the early adoption of this now common and crucial process in biology. [citation needed] In 1988, Bio-Synthesis helped in the synthesis and characterization of a new class of peptides with novel antimicrobial properties discovered at the NIH.
A synthetic primer may also be referred to as an oligo, short for oligonucleotide. DNA polymerase (responsible for DNA replication) enzymes are only capable of adding nucleotides to the 3’-end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary strand. [ 1 ]
Oligo may refer to: Oligomer , as an abbreviation for the general term, or specifically for oligonucleotide , oligopeptide , oligosaccharide , or oligoester . OLIGO Primer Analysis Software
Handguns and rifles illegally modified with devices called “Glock switches” to become machine guns have proliferated, officials say. The devices are tiny, cheap and easy to install.
Early attempts at mutagenesis using radiation or chemical mutagens were non-site-specific, generating random mutations. [2] Analogs of nucleotides and other chemicals were later used to generate localized point mutations, [3] examples of such chemicals are aminopurine, [4] nitrosoguanidine, [5] and bisulfite. [6]
The result is a stem-loop primer that excludes annealing involving shorter overlaps, but permits annealing of the primer to its fully complementary sequence in the target. Chimeric primers: some DNA bases in the primer are replaced with RNA bases, creating a chimeric sequence. The melting temperature of a chimeric sequence with another chimeric ...