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English: This is a diagram of the basic steps of a Ziehl-Neelsen (Acid Fast) staining procedure File:Basic steps of acid fast staining procedure.svg is a vector version of this file. It should be used in place of this PDF file when not inferior.
Mechanism of acid-fast staining in acid-fast cells and non-acid-fast cell [24] [25] [26] The mechanism of action of the Ziehl-Neelsen stain is not completely understood, but it is thought to involve a chemical reaction between the acidic dyes and the cell walls of the bacteria .
Franz Ziehl introduced the carbol fuchsin stain for the tubercle bacillus in 1882. With pathologist Friedrich Neelsen (1854–1898), he developed the Ziehl–Neelsen stain , [ 1 ] also known as the acid-fast stain, which is used to identify acid-fast bacteria .
[1] [2] Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast. [ 2 ] The mechanisms of acid-fastness vary by species although the most well-known example is in the genus Mycobacterium , which includes the species ...
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A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
Chinua Achebe was born on 16 November 1930 and baptised Albert Chinụalụmọgụ Achebe. [ 1 ] [ a ] His father, Isaiah Okafo Achebe, was a teacher and evangelist, and his mother, Janet Anaenechi Iloegbunam, was the daughter of a blacksmith from Awka , [ 3 ] a leader among church women, and a vegetable farmer.
In solution with phenol (also called carbolic acid) as an accentuator [17] it is called carbol fuchsin and is used for the Ziehl–Neelsen and other similar acid-fast staining of the mycobacteria which cause tuberculosis, leprosy etc. [18] Basic fuchsine is widely used in biology to stain the nucleus, and is also a component of Lactofuchsin ...