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Nicking can be used to dissipate the energy held up by intersecting states. The nicks allow the DNA to take on a circular shape. [2] The diagram shows the effects of nicks on intersecting DNA forms. A plasmid is tightly wound into a negative supercoil (a). To release the intersecting states, the torsional energy must be released by utilizing ...
A nicking enzyme (or nicking endonuclease) is an enzyme that cuts only one strand of a double-stranded DNA or RNA molecule [1] at a specific recognition nucleotide sequence known as the restriction site. Such enzymes hydrolyze (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved. [2] [3]
Nicking Enzyme Amplification Reaction (NEAR) is a method for in vitro DNA amplification like the polymerase chain reaction (PCR). NEAR is isothermal, replicating DNA at a constant temperature using a polymerase (and nicking enzyme ) to exponentially amplify the DNA at a temperature range of 55 °C to 59 °C.
Arteriovenous nicking, also known as AV nicking, is the phenomenon where, on examination of the eye, a small artery is seen crossing a small vein , which results in the compression of the vein with bulging on either side of the crossing.
The Free Dictionary: triangular swelling corresponding to the outline of the scapula Coombs test: Robin Coombs: hematology: hemolytic anemia: Coons fluorescent antibody method: Albert Coons: immunology: Albert Coons at National Academies Press: detection of antibodies by fluorescence microscopy using fluorescein-labelled antibodies
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Based on the properties of intercalating molecules, i.e. fluorescing upon binding to DNA and unwinding of DNA base-pairs, in 2016, a single-molecule technique has been introduced to directly visualize individual plectonemes along supercoiled DNA [5] which would further allow to study the interactions of DNA processing proteins with supercoiled DNA.
Signs of damage to the retina caused by hypertension include: [citation needed]. Laser Doppler imaging of the papilla of a patient with hypertension. Arteriolar changes, such as generalized arteriolar narrowing, focal arteriolar narrowing, arteriovenous nicking, changes in the arteriolar wall (arteriosclerosis) and abnormalities at points where arterioles and venules cross.