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Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phosphocreatine kinase, is an enzyme (EC 2.7.3.2) expressed by various tissues and cell types. CK catalyses the conversion of creatine and uses adenosine triphosphate (ATP) to create phosphocreatine (PCr) and adenosine diphosphate (ADP).
The energy used by human cells in an adult requires the hydrolysis of 100 to 150 mol/L of ATP daily, which means a human will typically use their body weight worth of ATP over the course of the day. [30] Each equivalent of ATP is recycled 1000–1500 times during a single day (150 / 0.1 = 1500), [29] at approximately 9×10 20 molecules/s. [29]
The adenylate energy charge is an index used to measure the energy status of biological cells.. ATP or Mg-ATP is the principal molecule for storing and transferring energy in the cell : it is used for biosynthetic pathways, maintenance of transmembrane gradients, movement, cell division, etc...
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The ATP generated in this process is made by substrate-level phosphorylation, which does not require oxygen. Fermentation is less efficient at using the energy from glucose: only 2 ATP are produced per glucose, compared to the 38 ATP per glucose nominally produced by aerobic respiration. Glycolytic ATP, however, is produced more quickly.
Steps 1 and 3 require the input of energy derived from the hydrolysis of ATP to ADP and P i (inorganic phosphate), whereas steps 7 and 10 require the input of ADP, each yielding ATP. [7] The enzymes necessary to break down glucose are found in the cytoplasm , the viscous fluid that fills living cells, where the glycolytic reactions take place.
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