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Golden Gate assembly involves digesting DNA sequences containing a type IIS restriction enzyme cut site and ligating them together. Golden Gate Cloning or Golden Gate assembly [1] is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase. [2]
The development of the Golden Gate assembly methods and its variants has allowed researchers to design tool-kits to speed up the synthetic biology workflow. For example, EcoFlex was developed as a toolkit for E. Coli that uses the MoClo standard for its DNA parts, while a similar toolkit has also been developed for engineering the Chlamydomonas ...
Cassette mutagenesis via Golden Gate Assembly. Cassette mutagenesis is a type of site-directed mutagenesis that uses a short, double-stranded oligonucleotide sequence (gene cassette) to replace a fragment of target DNA. It uses complementary restriction enzyme digest ends on the target DNA and gene cassette to achieve specificity.
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Daniel Voytas is an American geneticist who is Professor of Genetics, Cell Biology and Development at the University of Minnesota [1] and Director of the Center for Precision Plant Genomics. [2]
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The Silver Veil and the Golden Gate is a late period, coastal landscape painting by American Impressionist Childe Hassam. Completed in 1914 during one of his visits to California, the piece depicts the Golden Gate Strait, a narrow passage connecting the San Francisco Bay to the Pacific Ocean , as seen near Sausalito .