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G-banding, G banding or Giemsa banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. It is the most common chromosome banding method. [ 1 ] It is useful for identifying genetic diseases (mainly chromosomal abnormalities ) through the photographic representation of the entire chromosome ...
Only 3 bits are used for the red channel in the left two images, 8 bits in the right image. Colour banding is a subtle form of posterization in digital images, caused by the colour of each pixel being rounded to the nearest of the digital colour levels. While posterization is often done for artistic effect, colour banding is an undesired artifact.
That is, colors that are adjacent appear to overlap; a secondary color is observed. Some assays are designed so that the secondary color will be present or absent in cases of interest. An example is the detection of BCR/ABL translocations, where the secondary color indicates disease. This variation is often called double-fusion FISH or D-FISH.
For artistic effect, most image editing programs provide a posterization feature, or photographic processes may be used. Unwanted posterization, also known as banding, may occur when the color depth, sometimes called bit depth, is insufficient to accurately sample a continuous gradation of color tone. As a result, a continuous gradient appears ...
The skin is from a patient with systemic lupus erythematosus and shows IgG deposit at two different places: The first is a band-like deposit along the epidermal basement membrane ("lupus band test" is positive). The second is within the nuclei of the epidermal cells (anti-nuclear antibodies).
A standard test image is a digital image file used across different institutions to test image processing and image compression algorithms. By using the same standard test images, different labs are able to compare results, both visually and quantitatively. The images are in many cases chosen to represent natural or typical images that a class ...
A separation artifact in top image makes the tumor look incompletely excised, but the next microtomy level (bottom image) shows a surgical margin of connective tissue. Stacking of cells on top of each other gives a dark look, and in this breast tissue it may mimic microcalcifications.
R-banding is the reverse of G-banding (the R stands for "reverse"). The dark regions are euchromatic (guanine-cytosine rich regions) and the bright regions are heterochromatic (thymine-adenine rich regions). C-banding: Giemsa binds to constitutive heterochromatin, so it stains centromeres. The name is derived from centromeric or constitutive ...