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Nitrilotriacetic acid (NTA) is the aminopolycarboxylic acid with the formula N(CH 2 CO 2 H) 3. It is a colourless solid. It is a colourless solid. Its conjugate base nitrilotriacetate is used as a chelating agent for Ca 2+ , Co 2+ , Cu 2+ , and Fe 3+ .
Examples of methods for adding polyhistidine tags. (A) The polyhistidine tag is added by inserting the DNA encoding a protein of interest in a vector that has the tag ready to fuse at the C-terminus. (B) The polyhistidine tag is added using primers containing the tag coding sequence as an overhang on the forward primer. After PCR amplification ...
Further substitution gives nitrilotriacetic acid, NTA, which is a tetradentate ligand. [3] These compounds can be described as aminopolycarboxylates. Related ligands can be derived from other amino acids other than glycine, notably aspartic acid. Binding of a metal complex by the iminodiacetate anion
Nitrilotriacetic acid Index of chemical compounds with the same molecular formula This set index page lists chemical structure articles associated with the same molecular formula .
Nitrilotriacetonitrile (NTAN) is a precursor for nitrilotriacetic acid (NTA, a biodegradable complexing agent and building block for detergents), for tris(2-aminoethyl)amine (a tripodal tetradentate chelating agent known under the abbreviation tren) and for the epoxy resin crosslinker aminoethylpiperazine.
Knowing the structure of a similar homologous sequence (for example a member of the same protein family) allows highly accurate prediction of the tertiary structure by homology modeling. If the full-length protein sequence is available, it is possible to estimate its general biophysical properties, such as its isoelectric point.
The different types of lipid-linked oligosaccharide (LLO) precursor produced in different organisms.. N-linked glycosylation is the attachment of an oligosaccharide, a carbohydrate consisting of several sugar molecules, sometimes also referred to as glycan, to a nitrogen atom (the amide nitrogen of an asparagine (Asn) residue of a protein), in a process called N-glycosylation, studied in ...
It is produced during the breakdown of glucose, fatty acids, and amino acids, and is used in the synthesis of many other biomolecules, including cholesterol, fatty acids, and ketone bodies. Acetyl-CoA is also a key molecule in the citric acid cycle , which is a series of chemical reactions that occur in the mitochondria of cells and is ...