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Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...
It is a component of Papanicolaou stains together with eosin Y and bismarck brown Y. [1] In pap smears, Light Green SF confers a blue staining for the cytoplasm of active cells such as columnar cells, parabasal squamous cells, and intermediate squamous cells. [3] It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm.
Papanicolaou stain (also Papanicolaou's stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. [ 1 ] [ 2 ] [ 3 ] The Papanicolaou stain is one of the most widely used stains in cytology , [ 1 ] where it is used to aid pathologists in making a diagnosis.
Another common variant is the Masson trichrome & Verhoeff stain, which combines the Masson trichrome stain and Verhoeff's stain. [2] This combination is useful for the examination of blood vessels ; the Verhoeff stain highlights elastin (black) and allows one to easily differentiate small arteries (which typically have at least two elastic ...
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Bismarck brown Y stains acid mucins to yellow color. It also stains mast cell granules brown. [3] It can be used with live cells. It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA. It was more common in the past ...
Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears , urine samples, and bone marrow aspirates , which are examined under a light microscope .
Safranin (Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage, [2] mucin and mast cell granules.