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The tubes often carry out a series of calibrations of solutions of increasing concentrations, which functions as a comparative scale. To minimize differences in the subjective impression of the color of the solution of the substance to be analyzed, cylinders of a series should have the same characteristics - height, diameter, and thickness of ...
Reagent testers might show the colour of the desired substance while not showing a different colour for a more dangerous additive. [2] For this reason it is essential to use multiple different tests to show all adulterants.
Chemical tests use reagents to indicate the presence of a specific chemical in an unknown solution. The reagents cause a unique reaction to occur based on the chemical it reacts with, allowing one to know what chemical is in the solution. An example is Heller's test where a test tube
The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis . Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream.
These tubes are commonly sealed with a rubber stopper and often have a specific additive placed in the tube with the stopper color indicating the additive. For example, a blue-top tube is a 5 ml test tube containing sodium citrate as an anticoagulant, used to collect blood for coagulation and glucose-6-phosphate dehydrogenase testing. [5]
The Biuret assay utilizes a biuret reagent which turns purple in the presence of proteins due to the chelation of copper salts in an alkaline solution. [4] Enzyme linked immunoassays use enzyme-complexed-antibodies to detect antigens. Binding of the antibody is often inferred from the color change of reagents such as TMB.
These tubes are usually made of glass, but can also be made of plastic or metal. They vary in size and purpose. Laboratory sample tubes must not be confused with glass tubing, which can be utilized to carry fluid between laboratory equipment. Example laboratory sample tubes are listed below: Ignition tube; Test tube; Boiling tube; NMR tube
The Ehrlich reagent is similar to a number of other indole tests: The van Urk reagent, which uses 0.125 g of p-DMAB, 0.2 mL of ferric chloride solution (25 mg/mL) in 100 mL of 65% sulfuric acid. [9] [10] [11] This is sometimes referred to as the Hofmann reagent or p-DMAB-TS (Test Solution) and gives slightly different colours with different ...
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