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Saccharomyces cerevisiae (/ ˌ s ɛr ə ˈ v ɪ s i. iː /) (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungal microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times.
Saccharomyces cerevisiae was the first eukaryotic organism to have its complete genome sequence determined.. This list of "sequenced" eukaryotic genomes contains all the eukaryotes known to have publicly available complete nuclear and organelle genome sequences that have been sequenced, assembled, annotated and published; draft genomes are not included, nor are organelle-only sequences.
The Saccharomyces Genome Database (SGD) is a scientific database of the molecular biology and genetics of the yeast Saccharomyces cerevisiae, which is commonly known as baker's or budding yeast. [1] Further information is located at the Yeastract curated repository.
An upstream activating sequence or upstream activation sequence (UAS) is a cis-acting regulatory sequence found in yeast like Saccharomyces cerevisiae. It is distinct from the promoter and increases the expression of a neighbouring gene .
In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo.It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 μ plasmid of baker's yeast ...
The HO gene is a tightly regulated haploid-specific gene that is only activated in haploid cells during the G 1 phase of the cell cycle. [59] The protein encoded by the HO gene is a DNA endonuclease , which physically cleaves DNA, but only at the MAT locus (due to the DNA sequence specificity of the HO endonuclease).
Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae, which is then ligated into a bacterial plasmid. By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking.
Unlike mammals, yeast (Saccharomyces cerevisiae) do not have several isoforms of FMO, but instead only have one called yFMO. This enzyme does not accept xenobiotic compounds. Instead, yFMO helps to fold proteins that contain disulfide bonds by catalyzing O 2 and NADPH-dependent oxidations of biological thiols , just like mammalian FMO's.