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Escherichia coli BL21(DE3) is a commonly used protein production strain of the E. coli bacterium. This strain combines several features that allow for excessive expression of heterologous proteins . It is derived from the B lineage of E. coli .
Escherichia coli (/ ˌ ɛ ʃ ɪ ˈ r ɪ k i ə ˈ k oʊ l aɪ /; commonly abbreviated E. coli) is a Gram-negative gammaproteobacterium commonly found in the lower intestine of warm-blooded organisms (endotherms). The descendants of two isolates, K-12 and B strain, are used routinely in molecular biology as both a tool and a model organism.
Enteroinvasive E. coli (EIEC) Enterohemorrhagic E. coli (EHEC) Enteroaggregative E. coli (EAEC) Uropathogenic E. coli (UPEC) Verotoxin-producing E. coli; E. coli O157:H7 is an enterohemorrhagic strain also 2006 North American E. coli outbreak; E. coli O104:H4, also 2011 E. coli O104:H4 outbreak; Escherichia coli O121; Escherichia coli O104:H21
From 2002 to 2010, a team at the Hungarian Academy of Science created a strain of Escherichia coli called MDS42, which is now sold by Scarab Genomics of Madison, WI under the name of "Clean Genome E. coli", [123] where 15% of the genome of the parental strain (E. coli K-12 MG1655) were removed to aid in molecular biology efficiency, removing IS ...
E. coli (EIEC) found only in humans Bloody or nonbloody EIEC infection causes a syndrome that is identical to shigellosis, with profuse diarrhea and high fever. Enterohemorrhagic E. coli (EHEC) found in humans, cattle, and goats Bloody or nonbloody The most infamous member of this pathotype is strain O157:H7, which causes bloody diarrhea and no ...
Infection with Escherichia coli and Salmonella can result from the consumption of contaminated food and polluted water. Both of these bacteria are well known for causing nosocomial (hospital-linked) infections, and often, these strains found in hospitals are antibiotic resistant because of adaptations to wide spread antibiotic use. [32]
Escherichia coli is most common species for prokaryotic strain engineering. Scientists have succeeded in establishing viable minimal genomes from which new strains can be developed. [9] These minimal strains provide a near guarantee that experiments on genes outside the minimal framework will not be effected by non-essential pathways.
Shigatoxigenic Escherichia coli (STEC) and verotoxigenic E. coli (VTEC) are strains of the bacterium Escherichia coli that produce Shiga toxin (or verotoxin). [ a ] Only a minority of the strains cause illness in humans.