Search results
Results from the WOW.Com Content Network
Phage display is also a widely used method for in vitro protein evolution (also called protein engineering). As such, phage display is a useful tool in drug discovery. It is used for finding new ligands (enzyme inhibitors, receptor agonists and antagonists) to target proteins.
Later improvements of antibody phage display technology enables the display of millions of different antibody fragments on the surface of filamentous phage (better known as antibody phage library) and subsequent selection of highly specific recombinant antibodies to any given target. This technology is widely exploited in pharmaceutical ...
The first step is to have phage display libraries prepared. This involves inserting foreign desired gene segments into a region of the bacteriophage genome, so that the peptide product will be displayed on the surface of the bacteriophage virion. The most often used are genes pIII or pVIII of bacteriophage M13. [5]
Phage Display: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. OCLC 43903550, ISBN 0-87969-740-7; Birge, E. A. 2000. Bacterial and Bacteriophage Genetics. Springer-Verlag, New York. OCLC 41273243, ISBN 0-387-23919-7; Stahl, F. W. 2000. We Can Sleep Later: Alfred D. Hershey and the Origins of Molecular Biology.
The International Committee for Enteric Phage Typing was established in 1947, and these phage typing methods were soon standardized. [21] Improvements to the specificity of phage typing schemes were made throughout the next few decades. In 1959, Callow improved her initial scheme to differentiate 34 types of Salmonella typhimurium with 29 ...
General laboratory stands, racks, filter paper, reagents, etc. Induction coils: as a source of high voltage electricity Cathode ray oscilloscope ' Recording kymograph: historically, used in human or animal experiments to measure and record data Long extension kymograph: historically, used in or human animal experiments to measure and record data
Main page; Contents; Current events; Random article; About Wikipedia; Contact us
The rescue of function of the mutant β-galactosidase by the α-peptide is called α-complementation. In this method of screening, the host E. coli strain carries the lacZ deletion mutant ( lacZΔM15 ) which contains the ω-peptide, while the plasmids used carry the lacZα sequence which encodes the first 59 residues of β-galactosidase, the α ...