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The I 9.2 and I 2.1 cell lines. The I 2.1 cell line is functionally defective for FADD and the I 9.2 cell line is functionally defective for caspase-8, both defective molecules being essential to apoptosis or necroptosis of cells. The D1.1 cell line does not express the CD4 molecule, an important co-receptor in the activation pathway of helper ...
CHO cells in suspension. A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. The ...
Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur. [1] Most vertebrate-derived cells (with the exception of hematopoietic cells) are anchorage dependent and require a two-dimensional monolayer to facilitate cell adhesion, spreading and replication. [2]
Immunological synapse between Jurkat T cell expressing GFP-actin (green) and Raji B cell stained with CMAC (blue). Synapse formation was induced by Staphylococcal enterotoxin E superantigen . In immunology , an immunological synapse (or immune synapse ) is the interface between an antigen-presenting cell or target cell and a lymphocyte such as ...
RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [ 2 ]
Schematic of cell adhesion. Cell adhesion is the process by which cells interact and attach to neighbouring cells through specialised molecules of the cell surface. This process can occur either through direct contact between cell surfaces such as cell junctions or indirect interaction, where cells attach to surrounding extracellular matrix, a gel-like structure containing molecules released ...
Many cell lines that are widely used for biomedical research have been contaminated and overgrown by other, more aggressive cells. For example, supposed thyroid lines were actually melanoma cells, supposed prostate tissue was actually bladder cancer, and supposed normal uterine cultures were actually breast cancer. [4]
A microcarrier is a support matrix that allows for the growth of adherent cells in bioreactors.Instead of on a flat surface, cells are cultured on the surface of spherical microcarriers so that each particle carries several hundred cells, and therefore expansion capacity can be multiplied several times over. [1]
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