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The nanofocusing technique can create a nanometer-scale "white" light source at the tip apex, which can be used to illuminate a sample at near-field for spectroscopic analysis. The interband optical transitions in individual single-walled carbon nanotubes are imaged and a spatial resolution around 6 nm has been reported. [28]
Piezospectroscopy (also known as photoluminescence piezospectroscopy) is an analytical technique that reveals internal stresses in alumina-containing materials, particularly thermal barrier coatings (TBCs).
Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase ) and a porous solid ...
The protein manufacturing cost remains high and there is a growing demand to develop cost efficient and rapid protein purification methods. Understanding the different protein purification methods and optimizing the downstream processing is critical to minimize production costs while maintaining the quality of acceptable standards of homogeneity. [2]
Photoluminescence is an important technique for measuring the purity and crystalline quality of semiconductors such as GaN and InP and for quantification of the amount of disorder present in a system. [7] Time-resolved photoluminescence (TRPL) is a method where the sample is excited with a light pulse and then the decay in photoluminescence ...
The principle of tandem-affinity purification of multiprotein complexes is not limited to the combination of CBP and Protein A tags used in the original work by Rigaut et al. (1999). For example, the combination of FLAG- and HA-tags has been used since 2000 by the group of Nakatani [10] [11] to purify numerous protein complexes from mammalian ...
With fluorescence correlation spectroscopy, one protein is labeled with a fluorescent dye and the other is left unlabeled. The two proteins are then mixed and the data outputs the fraction of the labeled protein that is unbound and bound to the other protein, allowing you to get a measure of K D and binding affinity. You can also take time ...
Photoluminescence excitation (abbreviated PLE) is a specific type of photoluminescence and concerns the interaction between electromagnetic radiation and matter.It is used in spectroscopic measurements where the frequency of the excitation light is varied, and the luminescence is monitored at the typical emission frequency of the material being studied.
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