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The chloride ion is a well known quencher for quinine fluorescence. [2] [3] [4] Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence. Quenching is made use of in optode sensors; for instance the quenching effect of oxygen on certain ruthenium complexes allows the measurement of oxygen saturation in
In a reporter – quencher system the dye nonradiatively (without light) transfers energy to the quencher. This returns the dye to the ground state and generates the quencher excited state. The quencher then returns to the ground state through emissive decay (fluorescence) or nonradiatively (dark quenching). In nonradiative or dark decay ...
The role of quenchers is to absorb energy from a fluorophore and to re-emit the energy in the form of either heat (dark quenchers) or visible light (fluorescent quenchers). The absorption range of BHQ1 is from 480 to 580 nm with maximum absorption at 534 nm. [1] [2] [3] [4]
For diffusion-limited quenching (i.e., quenching in which the time for quencher particles to diffuse toward and collide with excited particles is the limiting factor, and almost all such collisions are effective), the quenching rate coefficient is given by = /, where is the ideal gas constant, is temperature in kelvins and is the viscosity of ...
Relaxation from an excited state can also occur through collisional quenching, a process where a molecule (the quencher) collides with the fluorescent molecule during its excited state lifetime. Molecular oxygen (O 2) is an extremely efficient quencher of fluorescence because of its unusual triplet ground state.
Fluorescent chloride sensors are used for chemical analysis. The discoveries of chloride (Cl − ) participations in physiological processes stimulates the measurements of intracellular Cl − in live cells and the development of fluorescent tools referred below.
Red No. 3 does not pose a health risk to the general Canadian population at the levels set out in the List of Permitted Food Colours." Is Red Dye No. 3 Dangerous?
Degradation of the probe releases the fluorophore from it and breaks the proximity to the quencher, thus relieving the quenching effect and allowing fluorescence of the fluorophore. Hence, fluorescence detected in the quantitative PCR thermal cycler is directly proportional to the fluorophore released and the amount of DNA template present in ...