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Fungal yeast forms are inconsistently stained with Acid-fast stain which is considered a narrow spectrum stain for fungi. [21] In a study on acid-fastness of fungi, [ 22 ] 60% of blastomyces and 47% of histoplasma showed positive cytoplasmic staining of the yeast-like cells, and Cryptococcus or candida did not stain, and very rare staining was ...
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
Staphylococcus aureus is a gram-positive spherically shaped bacterium, a member of the Bacillota, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. It is often positive for catalase and nitrate reduction and is a facultative anaerobe, meaning that it can grow without oxygen. [1]
Mechanism of acid-fast staining in acid-fast cells and non-acid-fast cell [24] [25] [26] The mechanism of action of the Ziehl-Neelsen stain is not completely understood, but it is thought to involve a chemical reaction between the acidic dyes and the cell walls of the bacteria .
Although bacteria are traditionally divided into two main groups, gram-positive and gram-negative, based on their Gram stain retention property, this classification system is ambiguous as it refers to three distinct aspects (staining result, envelope organization, taxonomic group), which do not necessarily coalesce for some bacterial species.
Staphylococcus spp. also inhabit human skin, but they are facultative anaerobes. They ferment sugars, producing lactic acid as an end product. Many of these species produce carotenoid pigments, which color their colonies yellow or orange. Staphylococcus aureus is a major human pathogen. It can infect almost any tissue in the body, frequently ...
Staphylococcus species are facultative anaerobes (capable of growth both aerobically and anaerobically). [15] All species grow in the presence of bile salts. All species of Staphylococcus aureus were once thought to be coagulase-positive, but this has since been disproven. [16] [17] [18] Growth can also occur in a 6.5% NaCl solution. [15]
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.