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Hemolysis by Streptococcus seen on a plate Not only are the erythrocytes affected by hemolysins, but there are also some effects among other blood cells, such as leucocytes (white blood cells). Escherichia coli hemolysin is potentially cytotoxic to monocytes, lymphocytes and macrophages , leading them to autolysis and death.
A red blood cell in a hypotonic solution, causing water to move into the cell A red blood cell in a hypertonic solution, causing water to move out of the cell. Hemolysis or haemolysis (/ h iː ˈ m ɒ l ɪ s ɪ s /), [1] also known by several other names, is the rupturing of red blood cells (erythrocytes) and the release of their contents into surrounding fluid (e.g. blood plasma).
(left) α-hemolysis (S. mitis); (middle) β-hemolysis (S. pyogenes); (right) γ-hemolysis (non-hemolytic, S. salivarius) Hemolysis is the breakdown of red blood cells. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms. This is particularly useful in classifying ...
Variety of microbiological samples. A laboratory specimen is sometimes a biological specimen of a medical patient's tissue, fluids, or other samples used for laboratory analysis to assist in differential diagnosis or staging of a disease process. These specimens are often the most reliable method of diagnosis, depending on the ailment.
The sucrose lysis test is a diagnostic laboratory test used for diagnosing paroxysmal nocturnal hemoglobinuria (PNH), as well as for hypoplastic anemias and any hemolytic anemia with an unclear cause. [1] The test works by using sucrose, which creates a low ionic strength environment that allows complement to bind to red blood cells. [1]
In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial) [1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent ), and stains the nuclei of the ...
For analysis of biomarkers in blood serum samples, it is possible to do a pre-separation by free-flow electrophoresis that usually consists of a depletion of serum albumin protein. [6] This method enables greater penetration of the proteome via separation of a wide variety of charged or chargeable analytes, ranging from small molecules to cells.
Other plasma processing methods exist, but generally do not provide the resolution or purity of chromatographic methods. Two-phase liquid extraction may be performed using polyethylene glycol (PEG)-phosphate Aqueous two-phase systems, with a PEG-rich top layer and a phosphate-rich bottom layer. Although this method is somewhat useful for ...