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  2. DNA sequencing - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing

    DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and ...

  3. Sequencing - Wikipedia

    en.wikipedia.org/wiki/Sequencing

    DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.

  4. Nucleic acid sequence - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_sequence

    DNA sequencing is the process of determining the nucleotide sequence of a given DNA fragment. The sequence of the DNA of a living thing encodes the necessary information for that living thing to survive and reproduce. Therefore, determining the sequence is useful in fundamental research into why and how organisms live, as well as in applied ...

  5. Sanger sequencing - Wikipedia

    en.wikipedia.org/wiki/Sanger_sequencing

    Sanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on ... large DNA fragments that differ in length by only one nucleotide. ...

  6. Sequence analysis - Wikipedia

    en.wikipedia.org/wiki/Sequence_analysis

    The method used in this study, which is called the “Sanger method” or Sanger sequencing, was a milestone in sequencing long strand molecules such as DNA. This method was eventually used in the human genome project. [5] According to Michael Levitt, sequence analysis was born in the period from 1969 to 1977. [6]

  7. Single-molecule real-time sequencing - Wikipedia

    en.wikipedia.org/wiki/Single-molecule_real-time...

    The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity of the DNA polymerase at a single molecule level.

  8. Pyrosequencing - Wikipedia

    en.wikipedia.org/wiki/Pyrosequencing

    Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...

  9. Maxam–Gilbert sequencing - Wikipedia

    en.wikipedia.org/wiki/Maxam–Gilbert_sequencing

    Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides. [1] An example Maxam–Gilbert sequencing reaction.

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