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Virus quantification is counting or calculating the number of virus particles (virions) in a sample to determine the virus concentration. It is used in both research and development (R&D) in academic and commercial laboratories as well as in production situations where the quantity of virus at various steps is an important variable that must be monitored.
A plaque-forming unit (PFU) is a measure used in virology to describe the number of virus particles capable of forming plaques per unit volume. [1] It is a proxy measurement rather than a measurement of the absolute quantity of particles: viral particles that are defective or which fail to infect their target cell will not produce a plaque and thus will not be counted.
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Upon entering the cell, the virion disassembles and the genetic material from the virus takes control of the cell infrastructure, thus enabling the virus to replicate. [1] The genetic material ( core , either DNA or RNA , along with occasionally present virus core protein ) inside the virion is usually enclosed in a protection shell, known as ...
The phi X 174 (or ΦX174) bacteriophage is a single-stranded DNA virus that infects Escherichia coli. This virus was isolated in 1935 by Nicolas Bulgakov [1] in Félix d'Hérelle's laboratory at the Pasteur Institute, from samples collected in Paris sewers. Its characterization and the study of its replication mechanism were carried out from ...
Particle size is a notion introduced for comparing dimensions of solid particles (), liquid particles (), or gaseous particles ().The notion of particle size applies to particles in colloids, in ecology, in granular material (whether airborne or not), and to particles that form a granular material (see also grain size).
The structure of the virus was determined in 1985 using x-ray crystallography by researchers at Purdue University and the University of Wisconsin led by Michael Rossmann. The virus was crystallized forming cubic crystals with four virus particles in each unit cell ( space group P 2 1 3, no. 198), similar to a cubic close-packed arrangement.
Download as PDF; Printable version ... (crassvirus) 3D structure of the ΦcrAss001 virus particle. a, Molecular surface of the viral particle (virion), viewed from ...