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Ninhydrin is most commonly used to detect fingerprints in forensic cases, as the terminal amines of lysine residues in peptides and proteins sloughed off in fingerprints react with ninhydrin. [2] [3] Ninhydrin is a white solid that is soluble in ethanol and acetone. [1] Ninhydrin can be considered as the hydrate of indane-1,2,3-trione.
Indane-1,2,3-trione, which reacts readily with nucleophiles (including water). Whereas for most carbonyl compounds, a carbonyl form is more stable than a product of water addition (hydrate), ninhydrin forms a stable hydrate of the central carbon because of the destabilizing effect of the adjacent carbonyl groups.
Dragendorff's reagent is prepared by mixing a concentrated solution of potassium iodide with a solution of bismuth subnitrate in a diluted acid (acetic acid or tartaric acid, hydrochloric acid or sulfuric acid is rarely being used) as a low pH is mandatory for this reagent.
Ninhydrin (heating, amines and amino-acids) Acidic vanillin (heating, general reagent) Phosphomolybdic acid (no heating, general reagent) In the case of lipids, the chromatogram may be transferred to a polyvinylidene fluoride membrane and then subjected to further analysis, for example, mass spectrometry. This technique is known as far-eastern ...
Hydrindantin is an organic chemical thought to be involved with the ninhydrin test for amines. [2] References This page was last edited ...
Ninhydrin tests for ammonia or primary amines; Phosphate tests test for phosphate; The sodium fusion test tests for the presence of nitrogen, sulfur, and halides in a sample; The Zerewitinoff determination tests for any acidic hydrogen; The Oddy test tests for acid, aldehydes, and sulfides; Gunzberg's test tests for the presence of hydrochloric ...
Documents that have been subjected to high levels of humidity will not generally retain indentations. As a result, other forensic examinations involving the wetting of a document, such as fingerprint development using a ninhydrin solution, should not be done until after examination for indentations. [14] Preparation
Proline (symbol Pro or P) [4] is an organic acid classed as a proteinogenic amino acid (used in the biosynthesis of proteins), although it does not contain the amino group-NH 2 but is rather a secondary amine.