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Ninhydrin is most commonly used to detect fingerprints in forensic cases, as the terminal amines of lysine residues in peptides and proteins sloughed off in fingerprints react with ninhydrin. [2] [3] Ninhydrin is a white solid that is soluble in ethanol and acetone. [1] Ninhydrin can be considered as the hydrate of indane-1,2,3-trione.
Indane-1,2,3-trione, which reacts readily with nucleophiles (including water). Whereas for most carbonyl compounds, a carbonyl form is more stable than a product of water addition (hydrate), ninhydrin forms a stable hydrate of the central carbon because of the destabilizing effect of the adjacent carbonyl groups.
Some fingerprint reagents are specific, for example ninhydrin or diazafluorenone reacting with amino acids. Others such as ethyl cyanoacrylate polymerisation, work apparently by water-based catalysis and polymer growth. Vacuum metal deposition using gold and zinc has been shown to be non-specific, but can detect fat layers as thin as one molecule.
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Documents that have been subjected to high levels of humidity will not generally retain indentations. As a result, other forensic examinations involving the wetting of a document, such as fingerprint development using a ninhydrin solution, should not be done until after examination for indentations. [14] Preparation
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Ninhydrin (heating, amines and amino-acids) Acidic vanillin (heating, general reagent) Phosphomolybdic acid (no heating, general reagent) In the case of lipids, the chromatogram may be transferred to a polyvinylidene fluoride membrane and then subjected to further analysis, for example, mass spectrometry. This technique is known as far-eastern ...