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Ninhydrin (2,2-dihydroxyindane-1,3-dione) is an organic compound with the formula C 6 H 4 (CO) 2 C(OH) 2. It is used to detect ammonia and amines . Upon reaction with these amines, ninhydrin gets converted into deep blue or purple derivatives, which are called Ruhemann's purple.
Indane-1,2,3-trione, which reacts readily with nucleophiles (including water). Whereas for most carbonyl compounds, a carbonyl form is more stable than a product of water addition (hydrate), ninhydrin forms a stable hydrate of the central carbon because of the destabilizing effect of the adjacent carbonyl groups.
Hydrindantin is an organic chemical thought to be involved with the ninhydrin test for amines. [2] References This page was last edited ...
Schiff bases can be synthesized from an aliphatic or aromatic amine and a carbonyl compound by nucleophilic addition forming a hemiaminal, followed by a dehydration to generate an imine.
Recent research has been conducted on lawsone's potential applications in the forensic science field. Since lawsone shows many similarities with ninhydrin, the current reagent for latent fingerprint development, studies have been conducted to see if lawsone can be used in this field. As of now the research is inconclusive, but optimistic.
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Frederick Sanger. In 1945, Frederick Sanger described its use for determining the N-terminal amino acid in polypeptide chains, in particular insulin. [4] Sanger's initial results suggested that insulin was a smaller molecule than previously estimated (molecular weight 12,000), and that it consisted of four chains (two ending in glycine and two ending in phenylalanine), with the chains cross ...
Hawkinsin is ninhydrin positive (a common test to detect amino acids and proteins with a free -NH 2 group). It is found in elevated concentrations in the urine in hawkinsinuria, which is probably related to the depletion of glutathione and resulting high excretion of 5-oxoproline. [2]